Vitamin B6 - Pyridoxine

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Pyridoxine.JPG

Figure 1. Pyridoxine, C8H11NO3, MW=169,18 g/mol
 
Vitamin B6 occurs in foods as pyridoxine (PN), pyridoxamine (PM), pyridoxal (PL) and the corresponding phosphate esters (PNP, PMP and PLP). Also glycosylated forms may be present in foods.  
 
Synonyms for vitamin B6:

  • pyridoxine, adermin

 
Summary of the determination by HPLC

Summary of the determination by microbiological assay



Golden standards

There are two European Standards for the determination of vitamin B6. Methods are described in child pages where you can get by clicking the links below:

EN 14164:2008 Foodstuffs - Determination of vitamin B6 by HPLC

EN 14166:2009 Foodstuffs - Determination of vitamin B6 by microbiological assay

Proficiency testing schemes 

Here are listed some completed, on-going and/or upcoming proficiency testing schemes concerning vitamin B6.

DRRR - Deutsches Referenzbüro für Lebensmittel-Ringversuche und Referenzmaterialien

FAPAS

PTA - Proficiency Testing Australia: Report No. 651 Foor Proficiency Testing Program (Round 31) - Vitamins

Livsmedelsverket, National Food Agency of Sweden - Report 24 (2011) Vitamins in Foods - Round V-9

 Some upcoming proficiency testing schemes can be found in the EPTIS database.

Other methods available

  • AACC 86-31.01 Vitamin B6 Complex---Microbiological Method
  • AOAC 961.15 Vitamin B6 (Pyridoxine, Pyridoxal, Pyridoxamine) in Food Extracts
    • microbiological
  • AOAC 985.32 Vitamin B6 (Pyridoxine, Pyridoxal, Pyridoxamine) in Ready-To-Feed Milk-Based Infant Formula
    • microbiological
  • EN 14663:2005 Foodstuffs - Determination of vitamin B6 (including its glycosylated forms) by HPLC


 Literature

  •  see separate child page below

EuroFIR assistance to this method/guidelines