Summary - vitamin K

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EN 14148:2003 Foodstuffs - Determination of vitamin K1 by HPLC:

Scope

This European Standard specifies a method for the determination of vitamin K1 in foodstuffs by high performance liquid chromatography (HPLC). The determination of vitamin K1 content is carried out by measurement of reduced phylloquinone. The method has been validated for milk and infant formula, however laboratory experiences exist which show that the method is also applicable to other type of foodstuffs.

Principle

After enzymatic removal of fat from the sample vitamin K1 is determined in an appropriate sample solution by high performance liquid chromatography separation coupled with post-column reduction and subsequent fluorometric detection. Vitamin K1 isomers are quantified as a single unresolved peak with a C18 column.

Precision data