Summary - vitamin C

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EN 14130:2003 Foodstuffs - Determination of vitamin C by HPLC

Scope

This European Standard specifies an HPLC-method for the determination of vitamin C in foodstuffs. Vitamin C is the sum of L ( + ) ascorbic acid and dehydro L ( + ) ascorbic acid.

Principle

Vitamin C is extracted from the sample to be analysed using metaphosphoric acid solution. A reducing solution is used to transform dehydro L ( + ) ascorbic acid to L ( + ) ascorbic acid. Total L (+) ascorbic acid content is determined by HPLC with a UV detection at 265 nm.

Precision data