Summary - B2

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EN 14152:2003                 Food stuffs – Determination of vitamin B2 by HPLC:

Scope

This European Standard specifies a method for the determination of vitamin B2 in foodstuffs by high performance liquid chromatography (HPLC). The determination of vitamin B2 content is carried out by measurement of riboflavin.

Principle

Riboflavin in an appropriate sample solution is determined after acid hydrolysis followed by dephosphorylation using an enzymatic treatment by highs performance liquid chromatographic (HPLC) separation with a fluorometric detection.

Precision data

 

CRM 421
Milk powder

CRM 487
Pig liver

Repeatability standard deviation s r, mg/100 g

0,046

0,181

Repeatability coefficient of variation, %

3,2

1,7

Repeatability value r = [2,83 x s r], mg/100 g

0,130

0,511

Reproducibility standard deviation s R, mg/100 g

0,106

0,832

Reproducibility coefficient of variation, %

7,3

7,9

Reproducibility value r = [2,83 x s R], mg/100 g

0,30

2,35




Data derived from EN 14152:2003.