Summary - β-carotene

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EN 12823-2:2000 Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of Beta-carotene


Scope


This European Standard specifies a method for the determination of total β-carotene in foodstuffs by high performance liquid chromatography (HPLC).


Principle


Determination of the sum of β-carotene isomers in an appropriate sample solution by HPLC and spectrometric detection in the visible range. The extract obtained after saponification as described in EN 12823-1 may be used for quantification. Identification on the basis of the retention times, and determination by the external standard method using peak areas or peak heights.


Internal standards methods may also be used if the corresponding recovery tests have proven the same behaviour of the internal standard during the analysis as the analyte itself.


Presicion data from EN 12823-2:

 

Margarine

Vitamin drink

Pudding powder

Mixed vegetables

Repeatability standard deviation s r, mg/100 g

0,011

0,065

0,085

0,71

Repeatability coefficient of variation, %

4,5

2,9

5,6

3,9

Repeatability value r = [2,83 x s r], mg/100 g

0,032

0,19

0,24

2,0

Reproducibility standard deviation s R, mg/100 g

0,024

0,15

0,14

2,7

Reproducibility coefficient of variation, %

9,7

6,5

9,3

15

Reproducibility limit r = [2,8 x s R], mg/100 g

0,069

0,41

0,40

7,6